CCDC47 Antibody

货号: Ab093389
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 ExactAb™ ? ExactAb™ —— 阿拉丁强调精准和验证质量的抗体系列。适用于可靠、特异性经验证的抗体应用。 已验证 ? 已验证级 —— 发布前在特定应用中确认性能。当需要试剂按声称工作的书面保证时使用。 0.8 mg/mL
应用
IF/ICC, IHC, WB
宿主种属
兔(Rabbit)
种属反应性
人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型
Rabbit IgG
偶联
Unconjugated
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规格
库存
价格
数量
10μl
Ab093389-10μl
现货 Stock Image
¥524.90
50μL
Ab093389-50μl
现货 Stock Image
¥1,499.90
100μL
Ab093389-100μl
期货 Stock Image
¥2,999.90
1ml
Ab093389-1ml
期货 Stock Image
¥16,999.90
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为什么选择此级别

ExactAb™,已验证,无载体 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Rabbit anti Human CCDC47 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000

IHC: 1/50-1/100

Rabbit anti Human CCDC47 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions. Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein (By similarity). Within the PAT subcomplex, CCDC47 occludes the lateral gate of the SEC61 complex (By similarity). Involved in the regulation of calcium ion homeostasis in the ER. Required for proper protein degradation via the ERAD (ER-associated degradation) pathway. Has an essential role in the maintenance of ER organization during embryogenesis (By similarity).

规格

产品名称
CCDC47 Antibody
别名
卷曲螺旋结构域蛋白47抗体 | CCDC47抗体
英文别名
CCDC 47 antibody | CCDC47 antibody | Coiled coil domain containing 47 antibody | Coiled coil domain containing protein 47 antibody | Coiled-coil domain-containing protein 47 antibody | GK001 antibody | MSTP041 antibody | CCDC47 | CCD47_HUMAN antibody | TH
规格或纯度
ExactAb™, Validated, Carrier Free, 0.8 mg/mL
宿主种属
兔(Rabbit)
特异性
CCDC47
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Fusion protein of human CCDC47 (AA 264-483)
阳性对照
WB: K562, HeLa, HEK293, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified
浓度
0.8 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 40% Glycerol, 0.05% Sodium azide, pH 7.4
防腐剂
0.05% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
图片

CCDC47 Antibody (Ab093389) - Western Blot
All lanes: CCDC47 Antibody (Ab093389) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 58 kDa
Exposure time: 8.1 s

CCDC47 Antibody (Ab093389) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using CCDC47 Antibody (Ab093389). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

CCDC47 Antibody (Ab093389) - IF/ICC 
IF analysis of CCDC47 Antibody (Ab093389) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CCDC47 Antibody (Ab093389) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
应用
应用名称
稀释比例
IHC

1/50 - 1/100

IF/ICC

1/200

WB

1/500-1/1000

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

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📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

关联靶点(人)
CCDC47 Tbio 含卷曲螺旋结构域的蛋白 47(Coiled-coil domain-containing protein 47) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到2个结果

批号(Lot Number) 证书类型 货号
ZJ24F0910049 分析证书 Ab093389
ZJ24F0910050 分析证书 Ab093389
溶液计算器

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