Rabbit anti Human CCT2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-3 μg/mL
IHC: 5-30 μg/mL
IF/ICC: 2.5 μg/mL
Protein Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
WB: HeLa and MCF7 cell lysates. IHC: Human glioma, kidney, stomach, breast cancer, cerebrum, spleen and Porcine cerebrum tissues. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified + Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
CCT2 Antibody (Ab093602) - Western Blot All lanes: CCT2 Antibody (Ab093602) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 54 kDa Exposure time: 12.6 s
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Porcine Cerebrum Tissue Primary Ab: 20 μg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Sample: Human Cerebrum Tissue Primary Ab: 20 µg/mL CCT2 Antibody (Ab093602) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Sample: Human Spleen Tissue Primary Ab: 20 µg/mL CCT2 Antibody (Ab093602) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Sample: Human Breast cancer Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IF/ICC
IF analysis of CCT2 Antibody (Ab093602) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CCT2 Antibody (Ab093602) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).