Rabbit anti Human CD2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application
WB: 1/1000-1/2000 IHC: 1/100-1/200
IF/ICC:1/200
Protein Function
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Recombinant CD2 Antibody (Ab094160) - Western Blot All lanes: Recombinant CD2 Antibody (Ab094160) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 15.8 s
Recombinant CD2 Antibody (Ab094160) - IF/ICC IF analysis of Recombinant CD2 Antibody (Ab094160) in Jurkat cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized in 0.2% Triton X-100 for 5 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant CD2 Antibody (Ab094160) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).