该受体通过与 CD28 或 CTLA-4 结合,参与 T 淋巴细胞增殖和白细胞介素-2 生成所必需的成本刺激信号。可能在 T 细胞活化和天真 T 细胞成本刺激的早期事件中发挥关键作用,如 T 细胞在活化后 24 小时内决定免疫与否。同工酶 2 会干扰 CD86 簇的形成,因此是 T 细胞活化的负调控因子。
翻译后:多泛素化;由 MARCH8 促进,导致内吞和溶酶体降解。
宿主种属
兔(Rabbit)
特异性
CD86
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human CD86 (AA 5-60).
阳性对照
WB: Raji, HeLa, Jurkat, MCF7, SH-SY5Y, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa, K562 cells.
Recombinant CD86 Antibody (Ab095458) - Western Blot All lanes: Recombinant CD86 Antibody (Ab095458) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 73, 97 kDa The expression profile and molecular weight observed is mainly due to glycosylation and has been described in the literature (PMID:21896490). Exposure time: 0.9 s
Recombinant CD86 Antibody (Ab095458) - IHC Immunohistochemical analysis of paraffin-embedded human tonsil, using Recombinant CD86 Antibody (Ab095458) .
Recombinant CD86 Antibody (Ab095458) - IF/ICC Immunofluorescent analysis using Recombinant CD86 Antibody (Ab095458) at 1/50 dilution.
Recombinant CD86 Antibody (Ab095458) - IF/ICC Immunohistochemical analysis of paraffin-embedded human tonsil, using Recombinant CD86 Antibody (Ab095458) .
Recombinant CD86 Antibody (Ab095458) - IF/ICC Immunohistochemical analysis of paraffin-embedded human tonsil, using Recombinant CD86 Antibody (Ab095458) .
Recombinant CD86 Antibody (Ab095458) - IF/ICC Immunofluorescent analysis of K562 cells, using Recombinant CD86 Antibody (Ab095458) .