Rabbit anti Human Cdc20 Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 20.0 s
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 10.0 s
Cdc20 Antibody (Ab095575) - IHC Immunohistochemical analysis of CDC20 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the Cdc20 Antibody (Ab095575) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.