ExactAb™,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Cdk4 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 2.5 µg/mL
Protein Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Cdk4 Antibody (Ab095764) - Western Blot
All lanes: Cdk4 Antibody (Ab095764) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 34 kDa
Observed band size: 33kDa
Exposure time: 2.2 s
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Samples: Human Lymphoma Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Samples: Human Lung cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Sample: Human Breast cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Sample: Human Lymphoma Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Sample: Human Lung cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
5-20 µg/mL
0.01-2 µg/mL
2.5 µg/mL
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1011833 |
分析证书 | Ab095764 |
| ZJ23F1101529 |
分析证书 | Ab095764 |
| ZJ23F1101530 |
分析证书 | Ab095764 |
| ZJ25F0420067 |
分析证书 | Ab095764 |
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