Recombinant CDK5RAP1 Antibody (Ab095771) - Western Blot All lanes: Recombinant CDK5RAP1 Antibody (Ab095771) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 68 kDa Observed band size: 61 kDa Exposure time: 27.0 s
Recombinant CDK5RAP1 Antibody (Ab095771) - IHC Immunohistochemical analysis of CDK5RAP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant CDK5RAP1 Antibody (Ab095771) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CDK5RAP1 Antibody (Ab095771) - IF/ICC IF analysis of CDK5RAP1 Antibody (Ab095771) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CDK5RAP1 Antibody (Ab095771) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).