Rabbit anti Human Cdk9 Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
Recombinant Cdk9 Antibody (Ab095812) - Western Blot All lanes: Recombinant Cdk9 Antibody (Ab095812) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 43 kDa Observed band size: 40 kDa Exposure time: 21.0 s
Recombinant Cdk9 Antibody (Ab095812) - IF/ICC Immunocytochemistry analysis of Cdk9 (green) in HeLa using Recombinant Cdk9 Antibody (Ab095812), and cells were counterstained with DAPI (blue).
Recombinant Cdk9 Antibody (Ab095812) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Cdk9 Antibody (Ab095812). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.