Rabbit anti Human CDKN2A/p14ARF Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Relevance The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Recombinant CDKN2A/p14ARF Antibody (Ab095833) - Western Blot All lanes: Recombinant CDKN2A/p14ARF Antibody (Ab095833) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 16 kDa Exposure time: 100.0 s
Recombinant CDKN2A/p14ARF Antibody (Ab095833) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant CDKN2A/p14ARF Antibody (Ab095833) at 1/50 dilution.