Rabbit anti Human CDKN3 Antibody, Polyclonal, could be used for WB, IHC and so on. Application
WB: 1/500-1/2000 IHC: 1/100-1/300
IF/ICC: 1/200 Protein Function May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner.
CDKN3 Antibody (Ab095865) - Western Blot All lanes: CDKN3 Antibody (Ab095865) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 18, 24 kDa Exposure time: 4.5 s
CDKN3 Antibody (Ab095865) - IHC Immunohistochemical analysis of paraffin-embedded human Colon cancer. CDKN3 Antibody (Ab095865) was diluted at 1/200 (4° overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
CDKN3 Antibody (Ab095865) - IF/ICC IF analysis of CDKN3 Antibody (Ab095865) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CDKN3 Antibody (Ab095865) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).