ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Cell adhesion molecule 4 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50 - 1/100
IF/IC :1/50 - 1/200
Protein Function
Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.
Cell adhesion molecule 4 Antibody (Ab095999) - Western Blot
All lanes: Cell adhesion molecule 4 Antibody (Ab095999) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 56, 72 kDa
The expression profile and molecular weight observed, which is due to glycosylation.
Exposure time: 0.3 s
Cell adhesion molecule 4 Antibody (Ab095999) - IF/ICC
Immunofluorescent analysis of CADM4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Cell adhesion molecule 4 Antibody (Ab095999) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Cell adhesion molecule 4 Antibody (Ab095999) - IHC
Immunohistochemical analysis of CADM4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Cell adhesion molecule 4 Antibody (Ab095999) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
1/50 - 1/100
1/50 - 1/200
1/500-1/1000
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1012567 |
分析证书 | Ab095999 |
| ZJ24F1012568 |
分析证书 | Ab095999 |
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