Cellular Apoptosis Susceptibility/CSE1L Antibody (Ab096010) - Western Blot All lanes: Cellular Apoptosis Susceptibility/CSE1L Antibody (Ab096010) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 110 kDa Observed band size: 106 kDa Exposure time: 10.0 s
Cellular Apoptosis Susceptibility/CSE1L Antibody (Ab096010) - IHC Immunohistochemical analysis of Cellular Apoptosis Susceptibility/CSE1L staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Cellular Apoptosis Susceptibility/CSE1L Antibody (Ab096010) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Cellular Apoptosis Susceptibility/CSE1L Antibody (Ab096010) - IF/ICC Immunofluorescent analysis of Cellular Apoptosis Susceptibility/CSE1L staining in NIH/3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Cellular Apoptosis Susceptibility/CSE1L Antibody (Ab096010) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.