Rabbit anti Human CFA Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Tubulin-folding protein; involved in the early step of the tubulin folding pathway.
规格
产品名称
CFA Antibody
别名
微管蛋白折叠辅酶A(TBCA)抗体 | 终末脂肪酸抗体
英文别名
Chaperonin cofactor A antibody | Co chaperonin associated with a & b tubulin antibody | Cofactor A antibody | tbca antibody | TBCA_HUMAN antibody | TCP1 chaperonin cofactor A antibody | TCP1-chaperonin cofactor A antibody | Tubulin cofactor a antibody | C
规格或纯度
ExactAb™, Validated, Carrier Free, 0.6 mg/mL
宿主种属
兔(Rabbit)
特异性
TBCA
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Fusion protein of human CFA (AA 1-108)
阳性对照
WB: HEK293, Jurkat, THP-1 and Jurkat cell lysates. IHC: Human thyroid cancer tissue. IF/ICC: HeLa cells.
CFA Antibody (Ab096146) - Western Blot All lanes: CFA Antibody (Ab096146) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 13 kDa Observed band size: 15 kDa Exposure time: 120.0 s
CFA Antibody (Ab096146) - IHC Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Tubulin Folding CFA Antibody (Ab096146). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
CFA Antibody (Ab096146) - IF/ICC IF analysis of CFA Antibody (Ab096146) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CFA Antibody (Ab096146) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).