CLCC1 Antibody (Ab096765) - Western Blot All lanes: CLCC1 Antibody (Ab096765) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 62 kDa Observed band size: 60, 191 kDa CLCC1 may form oligomers or multimeric structures. Many ion channels, including pore proteins in the endoplasmic reticulum, often form functional channels through oligomerization (PMID: 37142673). Exposure time: 120.0 s
CLCC1 Antibody (Ab096765) - IF/ICC Immunofluorescent analysis of CLCC1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with CLCC1 Antibody (Ab096765) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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