Rabbit anti Human CLIC4 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
Recombinant CLIC4 Antibody (Ab096857) - Western Blot All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 28 kDa Exposure time: 21.9 s
Recombinant CLIC4 Antibody (Ab096857) - Western Blot All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 28 kDa Exposure time: 35.0 s
Recombinant CLIC4 Antibody (Ab096857) - IF/ICC Immunocytochemistry analysis of CLIC4 (green) in HeLa using Recombinant CLIC4 Antibody (Ab096857), and cells were counterstained with DAPI (blue).
Recombinant CLIC4 Antibody (Ab096857) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant CLIC4 Antibody (Ab096857). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant CLIC4 Antibody (Ab096857) - Western Blot All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 28 kDa Exposure time: 0.5 s