Rabbit anti Human CLK2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/100-1/200
Protein Function
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
Post-translational
Autophosphorylates on all three types of residues.
CLK2 Antibody (Ab096876) - Western Blot All lanes: CLK2 Antibody (Ab096876) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 200.0 s
CLK2 Antibody (Ab096876) - IHC Immunohistochemical analysis of CLK2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the CLK2 Antibody (Ab096876) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.