Rabbit polyclonal to Connexin 43/GJA1 (phospho S368), could be used for WB, IHC and so on. Applications: WB: 1/500 - 1/2000 IHC: 1/100 - 1/300 Protein Function One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
规格
产品名称
Connexin 43/GJA1 (phospho S368) Antibody
别名
磷酸化Connexin 43蛋白抗体 | 磷酸化Connexin 43蛋白抗体
英文别名
Connexin 43(phospho-Ser368) | Connexin 43(phospho Ser368) | Connexin 43(phospho S368) | Connexin 43 | Connexin43v Cx 43v CX43 | DFNB38 | Gap junction 43 kDa heart protein | Connexin-43 | Gap junction alpha 1 protein | Gap junction protein alpha 1 43kDa (c
规格或纯度
Validated, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
GJA1 (phospho S368)
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
预测反应种属
Crab-eating macaque,Grivet,兔(Rabbit),狗(Dog)
免疫原
A synthetic peptide derived from human Connexin 43/GJA1 (phospho S368) (AA 332-381).
阳性对照
WB: K562 treated with 200ng/mL PMA for 10 min lysates. IHC: Human breast carcinoma tissue.
Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) - Western Blot All lanes: Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 31, 37 kDa The truncated isoforms are detected as distinct bands on the western blot lysate from the left ventricle of non-failing human heart tissue . In addition to 43 kDa full length isoform, the largest 3 isoforms (32 kDa, 29 kDa and 26 kDa in size) contain 2 transmembrane domains, part of intracellular loop, and the C-terminus. The smallest 3 isoforms are 20 kDa, 11 kDa, and 7 kDa in size and include part of the C-terminal tail with the 20 kDa isoform also containing part of the last transmembrane domain (PMID: 28576298). Exposure time: 26.2 s
Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) - IHC Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Connexin 43/GJA1 (phospho S368) Antibody (Ab097298). The picture on the right is blocked with the phosphor peptide.
ZJ24F1013112
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