Rabbit anti Human COX1/Cyclooxygenase 1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1-5 µg/mL IHC: 5-20 µg/mL Protein Function: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
Recombinant PTGS1 expressed in E.coli (AA 367-599).
阳性对照
WB: A431, THP-1 and C6 cell lysates. IHC: Human rectum, kidney tissues. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
COX1/Cyclooxygenase 1 Antibody (Ab097422) - Western Blot All lanes: COX1/Cyclooxygenase 1 Antibody (Ab097422) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 16.2 s
COX1/Cyclooxygenase 1 Antibody (Ab097422) - Western Blot All lanes: COX1/Cyclooxygenase 1 Antibody (Ab097422) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 69 kDa Observed band size: 72 kDa Exposure time: 5.0 s
COX1/Cyclooxygenase 1 Antibody (Ab097422) - IHC DAB staining on IHC Samples: Human Rectum Tissue Primary Ab: 10 µg/mL COX1/Cyclooxygenase 1 Antibody (Ab097422) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
COX1/Cyclooxygenase 1 Antibody (Ab097422) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL COX1/Cyclooxygenase 1 Antibody (Ab097422) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
COX1/Cyclooxygenase 1 Antibody (Ab097422) - IF/ICC IF analysis of COX1/Cyclooxygenase 1 Antibody (Ab097422) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with COX1/Cyclooxygenase 1 Antibody (Ab097422) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).