COX4I2 Antibody (Ab097457) - Western Blot All lanes: COX4I2 Antibody (Ab097457) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 17 kDa Exposure time: 120.0 s
COX4I2 Antibody (Ab097457) - IHC Immunohistochemical analysis of COX4-2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with COX4I2 Antibody (Ab097457) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
COX4I2 Antibody (Ab097457) - IF/ICC Immunofluorescent analysis of COX4-2 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with COX4I2 Antibody (Ab097457) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
应用
应用名称
稀释比例
IHC
1/50-1/200
IF/ICC
1/50-1/100
WB
1/500-1/2000
高级数据
关联靶点（人）
COX4I2Tbio细胞色素 c 氧化酶亚基 4 同工酶 2，线粒体(Cytochrome c oxidase subunit 4 isoform 2, mitochondrial)
(0 活性数据)
ZJ25F0218315
危险品化学品经营许可证（带存储）