Recombinant CPS1 Antibody (Ab097550) - Western Blot All lanes: Recombinant CPS1 Antibody (Ab097550) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 165kDa Observed band size: 156 kDa Exposure time: 10.0 s
Recombinant CPS1 Antibody (Ab097550) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant CPS1 Antibody (Ab097550). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant CPS1 Antibody (Ab097550) - IF/ICC
IF analysis of Recombinant CPS1 Antibody (Ab097550) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant CPS1 Antibody (Ab097550) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).