CSRP1 Antibody (Ab097983) - Western Blot All lanes: CSRP1 Antibody (Ab097983) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 22 kDa Exposure time: 15.0 s
CSRP1 Antibody (Ab097983) - Western Blot All lanes: CSRP1 Antibody (Ab097983) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 24 kDa Exposure time: 8.0 s
CSRP1 Antibody (Ab097983) - IHC Immunohistochemical analysis of CSRP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with CSRP1 Antibody (Ab097983) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CSRP1 Antibody (Ab097983) - IF/ICC Immunofluorescent analysis of CSRP1 staining in BV2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with CSRP1 Antibody (Ab097983) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).