Rabbit anti Human CCNB2 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Recombinant Cyclin B2/CCNB2 Antibody (Ab098427) - Western Blot All lanes: Recombinant Cyclin B2/CCNB2 Antibody (Ab098427) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa Observed band size: 44 kDa Exposure time: 100.3 s
Recombinant Cyclin B2/CCNB2 Antibody (Ab098427) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon using Recombinant Cyclin B2/CCNB2 Antibody (Ab098427). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
IF analysis of Recombinant Cyclin B2/CCNB2 Antibody (Ab098427) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Cyclin B2/CCNB2 Antibody (Ab098427) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).