Rabbit anti Human Cyclophilin F Antibody, Recombinant, could be used for WB, IHC and so on. Application:
WB: 1/500-1/1000 IHC: 1/500-1/1000
IF/ICC: 1/200
Protein Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Recombinant Cyclophilin F Antibody (Ab098522) - Western Blot All lanes: Recombinant Cyclophilin F Antibody (Ab098522) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 21 kDa Exposure time: 20.0 s
Recombinant Cyclophilin F Antibody (Ab098522) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Cyclophilin F Antibody (Ab098522). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Cyclophilin F Antibody (Ab098522) - IF/ICC IF analysis of Recombinant Cyclophilin F Antibody (Ab098522) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Cyclophilin F Antibody (Ab098522) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).