Responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It is involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants.
宿主种属
兔(Rabbit)
特异性
CYP2D6
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human CYP2D6.
阳性对照
WB: Mouse liver, Rat liver lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells.
Recombinant CYP2D6 Antibody (Ab098589) - Western Blot All lanes: Recombinant CYP2D6 Antibody (Ab098589) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 56 kDa Observed band size: 48 kDa Exposure time: 3.1 s
Recombinant CYP2D6 Antibody (Ab098589) - IHC Immunohistochemical analysis of Cytochrome P450 2D6 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant CYP2D6 Antibody (Ab098589) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Recombinant CYP2D6 Antibody (Ab098589) - IF/ICC Immunofluorescent analysis of Cytochrome P450 2D6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Recombinant CYP2D6 Antibody (Ab098589) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.