ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Cytokeratin 20 Antibody, Recombinant antibody (RR648), could be used for WB, IHC, ICC/IF, Flow and IP.
Application:
WB: 1/5000–1/20000
IHC: 1/800-1/1600
ICC/IF: 1/50-1/2000
Flow: 1/10-1/1000
IP: 1/50
Protein Function
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
Recombinant ACTA2 Antibody (Ab088446) - Western Blot
All lanes: Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa
Exposure time: 0.1 s
Recombinant Cytokeratin 20 Antibody (Ab098925) - IF/ICC
Recombinant Cytokeratin 20 Antibody (Ab098925) staining Cytokeratin 20 in HT-29 cells by IF/ICC (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant Cytokeratin 20 Antibody (Ab098925) (1/200) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500).
Recombinant Cytokeratin 20 Antibody (Ab098925) - Flow Cytometry
Overlay histogram showing HT-29 cells stained with Recombinant Cytokeratin 20 Antibody (Ab098925) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant Cytokeratin 20 Antibody (Ab098925) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Blue) was used as a control.
Recombinant Cytokeratin 20 Antibody (Ab098925) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of colonic adenocarcinoma tissue labelling Cytokeratin 20 with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant Cytokeratin 20 Antibody (Ab098925) - IP
Cytokeratin 20 was immunoprecipitated from 0.5 mg of HT-29 lysate with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/50 dilution.
2nd Ab: G&R HRP for IP 1/500
Lane 1: Recombinant Cytokeratin 20 Antibody (Ab098925) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Cytokeratin 20 Antibody (Ab098925) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 30.0 s.
1/800 - 1/1600
1/5000–1/20000
1/50-1/2000
1/10-1/1000
1/50
1/5000 - 1/20000
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0911326 |
分析证书 | Ab098925 |
| ZJ23F1001218 |
分析证书 | Ab098925 |
| ZJ23F1001219 |
分析证书 | Ab098925 |
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查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 → 查看 High Performance 级别指南 →
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