Rabbit anti Human DDX21 Antibody, Recombinant, could be used for WB, ICC/IF and so on. Application WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing.
规格
产品名称
Recombinant DDX21 Antibody
别名
重组 DDX21 抗体
英文别名
Ddx21 antibody | DDX21_HUMAN antibody | DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody | DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 antibody | DEAD box protein 21 antibody | EC 3.6.1.- antibody | Gu alpha antibody | Gu protein antibody | Gu-alpha antibody
规格或纯度
ExactAb™, Validated, 重组, 0.5 mg/mL
宿主种属
兔(Rabbit)
特异性
DDX21
种属反应性
人(Human)
免疫原
A synthetic peptide derived from human DDX21 (AA 248-260).
阳性对照
WB: HeLa, HEK293, Hep G2 and Jurkat cell lysates. ICC/IF: 293T cells.
Recombinant DDX21 Antibody (Ab099417) - Western Blot All lanes: Recombinant DDX21 Antibody (Ab099417) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 87 kDa Observed band size: 70-110 kDa DDX21 can bind to RNA and form specific motifs, which may result in a band shift phenomenon in a Western blot experiment. This shift occurs because the binding of RNA to DDX21 alters the size and charge of the protein-RNA complex, affecting its migration during gel electrophoresis. Exposure time: 34.8 s
Recombinant DDX21 Antibody (Ab099417) - IF/ICC Immunocytochemistry analysis of DDX21 (green) in 293T using Recombinant DDX21 Antibody (Ab099417) and DAPI (blue).