Rabbit anti Human Desmin Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/2000 IHC: 1/100-1/300 ICC/IF: 1/200-1/1000 Protein Function Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
Desmin Antibody (Ab099656) - Western Blot All lanes: Desmin Antibody (Ab099656) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 58, 54 kDa The expression profile and molecular weight observed may be due to post-translational modifications such as phosphorylation or glycosylation. Exposure time: 11.1 s
Desmin Antibody (Ab099656) - IF/ICC Immunofluorescence analysis of HeLa cells. Desmin Antibody (Ab099656) (green) was diluted at 1/200 (4 ℃ overnight). Goat Anti Rabbit AF488 Catalog: RS3211 was diluted at 1/1000 (room temperature, 50 min). DAPI (blue) 10 min.
Desmin Antibody (Ab099656) - IHC Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Desmin Antibody (Ab099656). The picture on the right is blocked with the synthesized peptide.