Rabbit anti Human Dishevelled/Dvl1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.01-2µg/mL
IHC: 5-20µg/mL
IF/ICC: 2.5µg/mL
Protein Function
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).
Dishevelled/Dvl1 Antibody (Ab099911) - Western Blot All lanes: Dishevelled/Dvl1 Antibody (Ab099911) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 100.0 s
Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 20 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Sample: Mouse Colon Tissue Primary Ab: 10 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Sample: Mouse Cerebrum Tissue Primary Ab: 10 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Sample: Mouse Liver Tissue Primary Ab: 10 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Sample: Mouse Kidney Tissue Primary Ab: 10 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Dishevelled/Dvl1 Antibody (Ab099911) - IF/ICC
IF analysis of Dishevelled/Dvl1 Antibody (Ab099911) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Dishevelled/Dvl1 Antibody (Ab099911) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).