ExactAb™,已验证,敲低验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human DNA Ligase IV/LIG4 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
Protein Function:
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) - Western Blot
All lanes: Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 98 kDa
Exposure time: 60.0 s
Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) - Western Blot
All lanes: Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 102 kDa
Exposure time: 9.2 s
Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) - Western Blot
All lanes use Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) at 1/1000 dilution for 1 hour at room temperature.
Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) - IF/ICC
Immunofluorescent analysis of Hela cells, using Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) at 1/50 dilution.
1/50-1/200
1/500-1/2000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0910263 |
分析证书 | Ab100073 |
| ZJ24F0910264 |
分析证书 | Ab100073 |
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查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 → 查看 KD Validation 级别指南 →
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