Rabbit anti Human UBA1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/1000
IHC：1/100-1/500
IF/ICC: 1/200 Protein Function: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
规格
产品名称
UBA1 Antibody
别名
泛素激活酶1抗体 | UBA1抗体
英文别名
EC 6.2.1.45 | Protein A1S9 | Ubiquitin-activating enzyme E1 | A1S9 antibody | A1S9 protein antibody | A1S9T and BN75 temperature sensitivity complementing antibody | A1S9T antibody | A1ST antibody | AMCX1 antibody | CFAP124 antibody | CTD-2522E6.1 antibod
规格或纯度
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
宿主种属
兔(Rabbit)
特异性
UBA1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A KLH conjugated synthetic peptide from the N-terminal region of human UBA1 (AA 1-30)
阳性对照
WB: K562, HeLa, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue. IF/ICC: HeLa cells.
UBA1 Antibody (Ab100767) Western Blot All lanes: UBA1 Antibody (Ab100767) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 118 kDa Observed band size: 116 kDa Exposure time: 6.5 s
UBA1 Antibody (Ab100767) - IHC UBA1 Antibody (Ab100767) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UBA1 Antibody (Ab100767) for immunohistochemistry. Clinical relevance has not been evaluated.
UBA1 Antibody (Ab100767) - IF/ICC
IF analysis of UBA1 Antibody (Ab100767) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with UBA1 Antibody (Ab100767) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F0303275
危险品化学品经营许可证（带存储）