Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
宿主种属
兔(Rabbit)
特异性
E2F1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human E2F1 (AA 365-400)
阳性对照
WB: HeLa, A375, NIH/3T3 and C6 cell lysates. IHC: Human ovary carcinoma tissue. IF/ICC: HeLa cells.
Recombinant E2F1 Antibody (Ab100782) - Western Blot All lanes: Recombinant E2F1 Antibody (Ab100782) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 66, 74 kDa Exposure time: 3.8 s
Recombinant E2F1 Antibody (Ab100782) - Western Blot All lanes use Recombinant E2F1 Antibody (Ab100782) at 1/1000 dilution for 1 hour at room temperature.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782).
Recombinant E2F1 Antibody (Ab100782) - IHC Immunohistochemical analysis of paraffin-embedded human ovary carcinoma, using Recombinant E2F1 Antibody (Ab100782).
ZJ25F0724457
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