Rabbit anti Human Eg5 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function: Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Recombinant Eg5 Antibody (Ab101062) - Western Blot All lanes: Recombinant Eg5 Antibody (Ab101062) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 119 kDa Observed band size: 121 kDa Exposure time: 5.9 s
Recombinant Eg5 Antibody (Ab101062) - IF/ICC Immunocytochemistry analysis of Eg5 (green) in HeLa using Recombinant Eg5 Antibody (Ab101062), and cells were counterstained with DAPI (blue).
Recombinant Eg5 Antibody (Ab101062) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Eg5 Antibody (Ab101062). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.