ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human eIF3A Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
Protein Function：
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
Recombinant eIF3A Antibody (Ab101307) - Western Blot
All lanes: Recombinant eIF3A Antibody (Ab101307) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 160 kDa
Observed band size: 188 kDa
Exposure time: 2.0 s
Recombinant eIF3A Antibody (Ab101307) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant eIF3A Antibody (Ab101307) at 1/50 dilution.
Recombinant eIF3A Antibody (Ab101307) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant eIF3A Antibody (Ab101307) at 1/50 dilution.
Recombinant eIF3A Antibody (Ab101307) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant eIF3A Antibody (Ab101307) at 1/150 dilution.
Recombinant eIF3A Antibody (Ab101307) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant eIF3A Antibody (Ab101307) at 1/200 dilution.
1/50-1/200
1/500-1/2000
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0910269 |
分析证书 | Ab101307 |
| ZJ24F0910270 |
分析证书 | Ab101307 |
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