Rabbit anti Human eIF3B Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Recombinant eIF3B Antibody (Ab101312) - Western Blot All lanes: Recombinant eIF3B Antibody (Ab101312) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 92 kDa Observed band size: 110 kDa Exposure time: 9.3 s
Recombinant eIF3B Antibody (Ab101312) - IF/ICC Immunocytochemistry analysis of Eif3b (green) in HeLa using Recombinant eIF3B Antibody (Ab101312), and cells were counterstained with DAPI (blue).