ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human EIF3H Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/100
Protein Function：
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
EIF3H Antibody (Ab101333) - Western Blot
All lanes: EIF3H Antibody (Ab101333) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 11.8 s
EIF3H Antibody (Ab101333) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using EIF3H Antibody (Ab101333). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
EIF3H Antibody (Ab101333) - IF/ICC
IF analysis of EIF3H Antibody (Ab101333) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with EIF3H Antibody (Ab101333) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
1/50 - 1/100
1/500-1/2000
1/200
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0303768 |
分析证书 | Ab101333 |
| ZJ24F0303769 |
分析证书 | Ab101333 |
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