eIF3l Antibody (Ab101338) - Western Blot All lanes: eIF3l Antibody (Ab101338) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 67 kDa Observed band size: 62 kDa Exposure time: 11.4 s
eIF3l Antibody (Ab101338) - IHC Immunohistochemical analysis of EIF3L staining in human Pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with eIF3l Antibody (Ab101338) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
eIF3l Antibody (Ab101338) - IF/ICC Immunofluorescent analysis of EIF3L staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with eIF3l Antibody (Ab101338) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ZJ25F0116449
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