Rabbit anti Human EIF4E2 Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/1000 IHC: 1/100-1/500 Protein function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
规格
产品名称
EIF4E2 Antibody
别名
EIF4E2蛋白抗体 | EIF4E2抗体
英文别名
4E LP | 4EHP | 4ELP | EIF 4E type 2 | eIF-4E type 2 | EIF4 E2 | EIF4E 2 | EIF4E HOMOLOGOUS PROTEIN | EIF4E like cap binding protein | EIF4E like protein 4E LP | EIF4E like protein 4ELP | EIF4E type 2 | EIF4E type 3 | eIF4E-like protein 4E-LP | EIF4EL3 | E
规格或纯度
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
宿主种属
兔(Rabbit)
特异性
EIF4E2
种属反应性
人(Human),小鼠(Mouse)
免疫原
Recombinant Human EIF4E2 Protein expressed in E.coli (AA 1-245)
阳性对照
WB: HEK293, Hep G2, HeLa, K562 and MCF7 cell lysates. IHC: Human stomach tissue.
EIF4E2 Antibody (Ab101387) - Western Blot All lanes: EIF4E2 Antibody (Ab101387) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa Observed band size: 30 kDa Exposure time: 9.5 s
EIF4E2 Antibody (Ab101387) - IHC EIF4E2 Antibody (Ab101387) staining EIF4E2 in human stomach tissue sections by Immunohistochemistry. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with EIF4E2 Antibody (Ab101387) (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.