Rabbit anti Human eIF4EBP1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
规格
产品名称
Recombinant eIF4EBP1 Antibody
别名
eIF4EBP1单克隆抗体 | eIF4EBP1单克隆抗体 | 重组 eIF4EBP1 抗体
英文别名
4EBP1 | 4E-BP1 | eIF4E-binding protein 1 | EIF4EBP1 | eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1 | eukaryotic translation initiation factor 4E-binding protein 1 | PHAS-I | PHAS-IMGC4316 | Phosphorylated heat- and acid-s
规格或纯度
ExactAb™, Validated, 重组, 0.2 mg/mL
宿主种属
兔(Rabbit)
特异性
EIF4EBP1
种属反应性
人(Human),仓鼠(Hamster),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human eIF4EBP1 (AA 16-33)
阳性对照
WB: Jurkat, HEK293, Hep G2, HeLa, MCF7, A431, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.
Recombinant eIF4EBP1 Antibody (Ab101403) - Western Blot All lanes: Recombinant eIF4EBP1 Antibody (Ab101403) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 13 kDa Observed band size: 18 kDa Exposure time: 78.9 s
Recombinant eIF4EBP1 Antibody (Ab101403) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant eIF4EBP1 Antibody (Ab101403). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant eIF4EBP1 Antibody (Ab101403) - IF/ICC IF analysis of Recombinant eIF4EBP1 Antibody (Ab101403) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant eIF4EBP1 Antibody (Ab101403) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).