Rabbit anti Human eIF-6 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/500
Protein Function
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
eIF-6 Antibody (Ab101442) - Western Blot All lanes: eIF-6 Antibody (Ab101442) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 26 kDa Exposure time: 52.9 s
eIF-6 Antibody (Ab101442) - IHC Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using eIF-6 Antibody (Ab101442). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
eIF-6 Antibody (Ab101442) - IF/ICC
IF analysis of eIF-6 Antibody (Ab101442) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with eIF-6 Antibody (Ab101442) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
ZJ24F0303344
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