Rabbit anti Human Emerin Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
Recombinant Emerin Antibody (Ab101562) - Western Blot All lanes: Recombinant Emerin Antibody (Ab101562) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 29 kDa Observed band size: 31, 33 kDa Exposure time: 54.5 s
Recombinant Emerin Antibody (Ab101562) - IF/ICC Immunocytochemistry analysis of Emerin (green) in HeLa using Recombinant Emerin Antibody (Ab101562), and cells were counterstained with DAPI (blue).
Recombinant Emerin Antibody (Ab101562) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Emerin Antibody (Ab101562). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.