Rabbit anti Human Entactin/NID Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500 - 1/1000 IHC: 1/50-1/100 Protein Function Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. Post-translational N- and O-glycosylated.
Recombinant Entactin/NID Antibody (Ab101762) - Western Blot All lanes: Recombinant Entactin/NID Antibody (Ab101762) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 136 kDa Observed band size: 52, 93, 144 kDa The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:3084254,12215539, 22952693). Exposure time:66.5 s
Recombinant Entactin/NID Antibody (Ab101762) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Entactin/NID Antibody (Ab101762).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.