Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
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Recombinant Epac1 Antibody (Ab101806) - Western Blot All lanes: Recombinant Epac1 Antibody (Ab101806) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 104 kDa Observed band size: 100 kDa Exposure time: 100.0 s
Recombinant Epac1 Antibody (Ab101806) - Western Blot All lanes: Recombinant Epac1 Antibody (Ab101806) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 104 kDa Observed band size: 95 kDa Exposure time: 150.0 s
Recombinant Epac1 Antibody (Ab101806) - Western Blot All lanes: Recombinant Epac1 Antibody (Ab101806) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 104 kDa Observed band size: 100 kDa Exposure time: 100.0 s
Recombinant Epac1 Antibody (Ab101806) - IF/ICC IF analysis of Recombinant Epac1 Antibody (Ab101806) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Epac1 Antibody (Ab101806) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).