ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human ERK5 Antibody, Recombinant, could be used for WB, IF, ICC and so on.
Application:
WB: 1/1000-1/2000
IF/ICC: 1/50-1/200
Protein Function
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
Post-translational
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
Recombinant ERK5 Antibody (Ab102241) - Western Blot
All lanes: Recombinant ERK5 Antibody (Ab102241) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 108 kDa
Exposure time: 158.9 s
Recombinant ERK5 Antibody (Ab102241) - Western Blot
All lanes: Recombinant ERK5 Antibody (Ab102241) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 108 kDa
Exposure time: 158.9 s
Recombinant ERK5 Antibody (Ab102241) - IF/ICC
Immunofluorescent analysis of HeLa, using Recombinant ERK5 Antibody (Ab102241) at 1/50 dilution.
1/50 - 1/200
1/1000 - 1/2000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0708405 |
分析证书 | Ab102241 |
| ZJ24F0708406 |
分析证书 | Ab102241 |
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