Rabbit anti Human Ezrin Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200 Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. Post-translational Phosphorylated by tyrosine-protein kinases.
Recombinant Ezrin Antibody (Ab102566) - Western Blot All lanes: Recombinant Ezrin Antibody (Ab102566) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 69 kDa Observed band size: 76 kDa Exposure time: 1.0 s
Recombinant Ezrin Antibody (Ab102566) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Ezrin Antibody (Ab102566). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Ezrin Antibody (Ab102566) - IF/ICC
IF analysis of Recombinant Ezrin Antibody (Ab102566) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Ezrin Antibody (Ab102566) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).