Rabbit anti Human FAK Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.5-2 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 3.25 µg/mL
Protein Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
WB: C6, NIH/3T3, K562, HUVEC and MCF7 cell lysates. IHC: Human Liver tissue, Human Thyroid tissue, Human Liver cancer tissue. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified and Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
FAK Antibody (Ab102794) - Western Blot All lanes: FAK Antibody (Ab102794) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 125 kDa Observed band size: 111 kDa Exposure time: 15.6 s
FAK Antibody (Ab102794) - Western Blot All lanes: FAK Antibody (Ab102794) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 125 kDa Observed band size: 111 kDa Exposure time: 15.6 s
FAK Antibody (Ab102794) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 20 µg/mL FAK Antibody (Ab102794) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
FAK Antibody (Ab102794) - IHC DAB staining on IHC Samples: Human Thyroid Tissue Primary Ab: 20 µg/mL FAK Antibody (Ab102794) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
FAK Antibody (Ab102794) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 20 µg/mL FAK Antibody (Ab102794) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
FAK Antibody (Ab102794) - IF/ICC
IF analysis of FAK Antibody (Ab102794) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with FAK Antibody (Ab102794) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).