Rabbit anti Human FE65 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function: Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
规格
产品名称
Recombinant FE65 Antibody
别名
淀粉样蛋白β前体蛋白结合蛋白B1(APBB1)抗体 | FE65抗体 | 重组FE65抗体
英文别名
APBB1_HUMAN antibody | FE 65 antibody | Fe65 protein antibody | Protein Fe65 antibody | RIR antibody | stat like protein antibody | RIR | FE65 | MGC:9072 | Adaptor protein FE65a2 antibody | Amyloid beta (A4) precursor protein binding family B member 1 ant
规格或纯度
ExactAb™, Validated, 重组, 0.3 mg/mL
宿主种属
兔(Rabbit)
特异性
FE65
种属反应性
人(Human)
免疫原
A synthetic peptide derived from human FE65 (AA 697-710)
阳性对照
WB: A549, SK-N-SH, U-2 OS and HEK293 cell lysates. IHC: Human lung cancer tissue. ICC/IF: 293T cells.
Recombinant FE65 Antibody (Ab103161) - Western Blot All lanes: Recombinant FE65 Antibody (Ab103161) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 77 kDa Observed band size: 70, 91 kDa Exposure time: 17.1 s
Recombinant FE65 Antibody (Ab103161) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant FE65 Antibody (Ab103161). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant FE65 Antibody (Ab103161) - IF/ICC Immunocytochemistry analysis of FE65 (green) in 293T using Recombinant FE65 Antibody (Ab103161) and DAPI (blue).