Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
宿主种属
兔(Rabbit)
特异性
FEN1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),猴(Monkey)
免疫原
A synthetic peptide derived from human FEN1
阳性对照
WB: Jurkat, A375, Raji, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. ICC/IF: MCF7 cells.
FEN1 Antibody (Ab103172) - Western Blot All lanes: FEN1 Antibody (Ab103172) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 11.9 s
FEN1 Antibody (Ab103172) - IF/ICC Immunofluorescent analysis of FEN1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 min and blocked with 3% BSA-PBS for 30 min at room temperature. Cells were probed with the FEN1 Antibody (Ab103172) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
FEN1 Antibody (Ab103172) - IHC Immunohistochemical analysis of FEN1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the FEN1 Antibody (Ab103172) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ZJ24F0809515
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