FFAR3/GPR41 Antibody (Ab103246) - Western Blot All lanes: FFAR3/GPR41 Antibody (Ab103246) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 35 kDa Exposure time: 28.1 s
FFAR3/GPR41 Antibody (Ab103246) - Western Blot All lanes: FFAR3/GPR41 Antibody (Ab103246) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 35 kDa Exposure time: 30 s
FFAR3/GPR41 Antibody (Ab103246) - IF/ICC Immunofluorescent analysis of GPR41 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with FFAR3/GPR41 Antibody (Ab103246) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.