Rabbit anti Human FHIT Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on. Application WB: 1/500-1/2000 IHC: 1/100 - 1/300 IF: 1/200 - 1/1000
规格
产品名称
FHIT Antibody
别名
脆性组氨酸三联体抗体
英文别名
fragile histidine triad | AP3A hydrolase | AP3A hydrolase fragile site 3p14.2 | AP3Aase | Bis 5' adenosyl triphosphatase | Dinucleosidetriphosphatase | FRA 3B | FRA3B | Fragile histidine triad gene | Fragile histidine triad protein | Tumor suppressor prot
规格或纯度
Validated, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
FHIT
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human FHIT (AA 81-130).
阳性对照
WB: Mouse kidney, A549 lysates. IHC: Human breast carcinoma, kidney tissues. IF/ICC: A549 cells.
FHIT Antibody (Ab103425) - Western Blot All lanes: FHIT Antibody (Ab103425) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 17 kDa Observed band size: 19 kDa Exposure time: 10.3 s
FHIT Antibody (Ab103425) - Western Blot All lanes: FHIT Antibody (Ab103425) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/10000 dilution Predicted band size: 17 kDa Observed band size: 19 kDa Exposure time: 38.6 s
FHIT Antibody (Ab103425) - IF/ICC Immunofluorescence analysis of A549 cells, using FHIT Antibody (Ab103425). The picture on the right is blocked with the synthesized peptide.
FHIT Antibody (Ab103425) - IHC Immunohistochemical analysis of paraffin-embedded Human kidney. FHIT Antibody (Ab103425) was diluted at 1:100 (4℃, overnight). High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200 (room temperature, 30 min).
FHIT Antibody (Ab103425) - IHC Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FHIT Antibody (Ab103425). The picture on the right is blocked with the synthesized peptide.