Rabbit anti Human Fibrillarin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/2000 IHC: 1/50-1/100 ICC/IF: 1/50-1/100 Protein Function: S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Recombinant Fibrillarin Antibody (Ab103454) - Western Blot All lanes: Recombinant Fibrillarin Antibody (Ab103454) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 2.1 s
Recombinant Fibrillarin Antibody (Ab103454) - Western Blot All lanes: Recombinant Fibrillarin Antibody (Ab103454) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 2.1 s
Recombinant Fibrillarin Antibody (Ab103454) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Fibrillarin Antibody (Ab103454).
Recombinant Fibrillarin Antibody (Ab103454) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Fibrillarin Antibody (Ab103454) at 1/50 dilution.
Recombinant Fibrillarin Antibody (Ab103454) - IHC Immunohistochemical analysis of paraffin-embedded human testis, using Recombinant Fibrillarin Antibody (Ab103454).